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KMID : 0923620130130010010
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Immune Network
2013 Volume.13 No. 1 p.10 ~ p.15
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Alum Directly Modulates Murine B Lymphocytes to Produce IgG1 Isotype
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Jin Bo-Ra
Kim Sun-Jin
Lee Jeong-Min
Kang Seong-Ho
Han Hye-Ju
Jang Young-Saeng
Seo Goo-Young
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Abstract
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Aluminum hydroxide (alum) is the most widely used adjuvant in human vaccines. Nevertheless, it is virtually unknown whether alum acts on B cells. In the present study, we explored the direct effect of alum on Ig expression by murine B cells in vitro. LPS-activated mouse spleen B cells were cultured with alum, and the level of isotype-specific Ig secretion, IgG1 secreting cell numbers, and Ig germ-line transcripts (GLT) were measured using ELISA, ELISPOT, and RT-PCR, respectively. Alum consistently enhanced total IgG1 production, numbers of IgG1 secreting cells, and GLT?1 expression. These results demonstrate that alum can directly cause IgG1 isotype switching leading to IgG1 production.
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KEYWORD
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IgG1, B lymphocyte, Isotype switch
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